Annotating Genes 1-36 in Gordonia Phage Bavilard

Department

Sciences & Mathematics

Format of Presentation

Poster

Research Category

STEM

Description

Gordonia phage Bavilard is a bacteriophage that attacks the host , Gordonia rubripertincta NRRL B-16540. It was first discovered under a tree in a soil sample by Indiana University Southeast, in Jeffersonville Indiana. The purpose of this research was to annotate all 72 genes in Bavilard and determine their functions. To accomplish this we used a website called PECAAN (Phage Evidence Collection and Annotation Network) and other databases like NCBI, HHPRED, CDD, Phagesdb. In the project we formed our own methodology to annotate each gene. After all the annotations were complete 23 genes functions were determined. The rest were labeled as a hypothetical protein. One notable protein discovered in this project was a Lysin A protein. In Gordina phage Bavilard there are two copies of Lysin A as well as Lysin B which are used by the bacteriophage to degrade the cell wall of the host after reproduction. Once the annotations were completed the genes were reviewed by Sea Phages and submitted to GenBank.

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Annotating Genes 1-36 in Gordonia Phage Bavilard

Gordonia phage Bavilard is a bacteriophage that attacks the host , Gordonia rubripertincta NRRL B-16540. It was first discovered under a tree in a soil sample by Indiana University Southeast, in Jeffersonville Indiana. The purpose of this research was to annotate all 72 genes in Bavilard and determine their functions. To accomplish this we used a website called PECAAN (Phage Evidence Collection and Annotation Network) and other databases like NCBI, HHPRED, CDD, Phagesdb. In the project we formed our own methodology to annotate each gene. After all the annotations were complete 23 genes functions were determined. The rest were labeled as a hypothetical protein. One notable protein discovered in this project was a Lysin A protein. In Gordina phage Bavilard there are two copies of Lysin A as well as Lysin B which are used by the bacteriophage to degrade the cell wall of the host after reproduction. Once the annotations were completed the genes were reviewed by Sea Phages and submitted to GenBank.