Department
Sciences & Mathematics
Format of Presentation
Poster
Research Category
STEM
Description
Extracellular vesicles (EV) are lipid-bound containers derived from the endosomal membrane network or from the extracellular membrane of cells. They can carry proteins, lipids, miRNAs, and other molecules, and may be involved in intercellular communication via membrane-associated proteins. Glioblastomas (GBM) are aggressive cancers of the brain and spinal cord derived from astrocytes. Extracellular vesicles derived from glioblastomas can make their way through the blood brain barrier and find their way to different bodily fluids, and so could serve as biomarkers for this cancer. We have isolated phage-display peptides from a commercial library of random phage-displayed peptides that will bind glioblastoma extracellular vesicles (GBM-EVs). We compared dose-response ELISA quantification of phage-display peptides bound to extracellular vesicles from glioblastomas to phage-mediated real-time immuno-PCR. Real-time immuno-PCR is a powerful technique that combines ELISA with the specificity and sensitivity of PCR. Both techniques had a hard time distinguishing GBM-EV-binding phage from control phage, but preliminary results indicated that real-time immuno-PCR had a higher sensitivity at low concentrations.
Recommended Citation
rudolf, jadelynn, "Phage Peptide Technology to Characterize Extracellular Vesicles in the Brain Tumors" (2023). Undergraduate Research Conference. 5.
https://athenacommons.muw.edu/urc/2023/poster/5
Phage Peptide Technology to Characterize Extracellular Vesicles in the Brain Tumors
Extracellular vesicles (EV) are lipid-bound containers derived from the endosomal membrane network or from the extracellular membrane of cells. They can carry proteins, lipids, miRNAs, and other molecules, and may be involved in intercellular communication via membrane-associated proteins. Glioblastomas (GBM) are aggressive cancers of the brain and spinal cord derived from astrocytes. Extracellular vesicles derived from glioblastomas can make their way through the blood brain barrier and find their way to different bodily fluids, and so could serve as biomarkers for this cancer. We have isolated phage-display peptides from a commercial library of random phage-displayed peptides that will bind glioblastoma extracellular vesicles (GBM-EVs). We compared dose-response ELISA quantification of phage-display peptides bound to extracellular vesicles from glioblastomas to phage-mediated real-time immuno-PCR. Real-time immuno-PCR is a powerful technique that combines ELISA with the specificity and sensitivity of PCR. Both techniques had a hard time distinguishing GBM-EV-binding phage from control phage, but preliminary results indicated that real-time immuno-PCR had a higher sensitivity at low concentrations.