Presentation Title

Oral Presentations: STEM

Presenter Information

Zithlaly Amezquita, Mississippi University for Women

Description

Each oral presentation of 15 minutes is followed by Q&A.

Comments

Zithlaly Amezquita, Mississippi University for Women

Endogenous CFTR Expression in Human Epithelial Cell Lines

Cystic Fibrosis Transmembrane-conductance Regulator, CFTR, is a large size glycoprotein that is present on the cell surface of many epithelial cells. There are several mutations that effect the function of CFTR. The most common mutation, dF508, causes a genetic disease called Cystic Fibrosis (CF). CF is common among the Caucasians of Northern European origin. The endogenous CFTR expression is difficult to detect by conventional western blotting methods. Using a newly developed western blotting method, I propose to detect the endogenous CFTR expression in human pancreatic cell lines, viz, Capan-l and CFPAC. By using human lung cell lines that have been transfected with the CFTR gene, I will also be comparing the endogenous CFTR expression to its exogenous expression.

Recommended Citation

Amezquita, Zithlaly, "Oral Presentations: STEM" (2022). Mississippi Undergraduate Honors Conference. 1.
https://athenacommons.muw.edu/muhc/2022/track51015amconcurrentsession2/1